WHY CHOOSE ZENIT G-SIGHT OVER A CLASSICAL CELL-BASED IIF ASSAY?
IIF assay is a low-cost, simple and rapid method for routine autoimmune screening; however, it has several disadvantages, which can be easily resolved with Zenit G Sight.
Classical cell-based IIF assay |
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Zenit G Sight system |
- Subjective analysis
- Limited number of samples
- Time-consuming sample screening
- Fluorochrome decay
- Organized archiving space required
- Difficult to exchange results with other labs
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- Objective quantification and pattern recognition
- High workload management
- Efficient automated +/- sera discrimination
- Digitized image data
- File storage and easy retrieval of previous reports
- Online access and data exchange
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ZENIT G-SIGHT IS:
- Valuable and accurate
giving clinicians a precious aid for diagnosis by providing a full quantitative/qualitative report on autoantibody serological status
- Reliable
providing results that are reproducible and comparable within and between laboratories
- Cost-effective
alleviating the heavy workload of laboratories with large sample numbers, maximizing efficiency and optimizing human resources
- Secure
safeguarding results and improving data management
WHAT ARE THE KEY FEATURES OF ZENIT G-SIGHT
Hardware components are fully controlled by software and mainly involved in the digital image acquisition process.
- Fluorescence microscope
Zenit G Sight microscope is equipped with a high-performance autofocus mechanism (5 to 13 focusing points per well) and supplied with two objectives (4x, 40x) on a revolving turret. Additional objectives can be provided on request (the revolving turret can accommodate 6 objectives).
- LED light source
LED illumination technology has numerous advantages over conventional light sources: for example it delivers highly focused light, generates little heat and is virtually maintenance free (long-life light source). Morevover, LED light sources do not photobleach FITC as fast as mercury lamps do.
HARDWARE COMPONENTS
- Scan stage
Zenit G Sight’s XY-motorized scan stage holds up to 5 slides (containing various number of wells) and is equipped with a barcode reader. For maximizing accuracy, a large area is scanned (from 1 to 220 fields per well), therefore the average scanning time for a 14-well slide may vary from 30 to 60 minutes.
- Digital camera
The high-sensitivity CCD camera of the Zenit G Sight system captures clear, high-contrast fluorescence images.