WHY CHOOSE ZENIT G-SIGHT OVER A CLASSICAL CELL-BASED IIF ASSAY?
IIF assay is a low-cost, simple and rapid method for routine autoimmune screening; however, it has several disadvantages, which can be easily resolved with Zenit G Sight.
Classical cell-based IIF assay
Zenit G Sight system
- Subjective analysis
- Limited number of samples
- Time-consuming sample screening
- Fluorochrome decay
- Organized archiving space required
- Difficult to exchange results with other labs
- Objective quantification and pattern recognition
- High workload management
- Efficient automated +/- sera discrimination
- Digitized image data
- File storage and easy retrieval of previous reports
- Online access and data exchange
ZENIT G-SIGHT IS:
- Valuable and accurate
giving clinicians a precious aid for diagnosis by providing a full quantitative/qualitative report on autoantibody serological status
providing results that are reproducible and comparable within and between laboratories
alleviating the heavy workload of laboratories with large sample numbers, maximizing efficiency and optimizing human resources
safeguarding results and improving data management
WHAT ARE THE KEY FEATURES OF ZENIT G-SIGHT
Hardware components are fully controlled by software and mainly involved in the digital image acquisition process.
- Fluorescence microscope
Zenit G Sight microscope is equipped with a high-performance autofocus mechanism (5 to 13 focusing points per well) and supplied with two objectives (4x, 40x) on a revolving turret. Additional objectives can be provided on request (the revolving turret can accommodate 6 objectives).
- LED light source
LED illumination technology has numerous advantages over conventional light sources: for example it delivers highly focused light, generates little heat and is virtually maintenance free (long-life light source). Morevover, LED light sources do not photobleach FITC as fast as mercury lamps do.
- Scan stage
Zenit G Sight’s XY-motorized scan stage holds up to 5 slides (containing various number of wells) and is equipped with a barcode reader. For maximizing accuracy, a large area is scanned (from 1 to 220 fields per well), therefore the average scanning time for a 14-well slide may vary from 30 to 60 minutes.
- Digital camera
The high-sensitivity CCD camera of the Zenit G Sight system captures clear, high-contrast fluorescence images.