HEp-2 CELL SUBSTRATE SLIDES
All our substrate slides locally distributed are compatible with the A. Menarini Diagnostics Zenit series platforms and can be assessed either visually with a fluorescence microscope or automatically with the A. Menarini Diagnostics interpretation system Zenit G Sight.
CHARACTERISTICS
|
|
Zenit HEp-2 from Menarini Diagnostics |
|
|
- Significant number of cells in mitosis
- Optimized cell morphology
- Large nucleus for easy pattern recognition
- Optimal substrate for the detection of antibodies targeting cytoplasmic antigens such as antigens associated with ribosomal P, tRNA synthetase (Jo-1), mitochondria and smooth muscle
- ANA positive control provided
- Substrate provided in accordance with CLSI standard guidelines
|
ORDERING INFORMATION
Our products are conveniently available in all parts of Europe through a network of local A. Menarini Diagnostics branches. Please contact your nearest branch for additional information and order placing.
DIAGNOSTIC SIGNIFIANCE OF ANTI-NUCLEAR ANTIBODIES
IIF staining pattern |
Nature of antigen |
Associated disease |
|
|
|
|
Homogeneous/Peripheral |
Deoxyribonucleoprotein
DNA |
SLE with renal involvement
SLE |
|
Nucleolar |
4S-6S RNA probably U3 RNA |
Scleroderma |
|
Speckled |
RNP
Sm
SS-A/SS-B
Scl-70 |
SLE or Mixed Connective Tissue Disease
SLE
SLE or Sjögren’s
Scleroderma |
|
Centromere |
Inner and outer plates of kinetochore |
CREST Syndrome |
|
Cytoplasmic/Mitochondrial |
70 kd mitochondrial protein |
primary biliary cirrhosis |
Anti-nuclear antibody (ANA) detection: Zenit G Sight vs visual assessment ANAs were determined by IIF using HEp-2 cells as a substrate. Tests were conducted blinded on 120 sera from patients with Connective Tissue Diseases or other autoimmune diseases and healthy subjects. Results were evaluated by three independent expert physician examiners and compared with evaluation by the Zenit G Sight automated interpretation system. Excellent agreement (100%) was obtained for the differentiation of positive and negative samples. Discrepancies were observed only in sera identified by Zenit G Sight system as “doubtful negative”: 8% of these were reported “positive speckled” by examiners. |